Komagataella pseudopastoris

Komagataella pseudopastoris
Scientific classification
Domain: Eukaryota
Kingdom: Fungi
Division: Ascomycota
Class: Pichiomycetes
Order: Pichiales
Family: Pichiaceae
Genus: Komagataella
Species:
K. pseudopastoris
Binomial name
Komagataella pseudopastoris
(Dlauchy, Tornai-Leh., Fülöp & G.Péter, 2003) Kurtzman, 2005
Synonyms

Pichia pseudopastoris

Komagataella pseudopastoris is an ascomycete fungi existing as a yeast.

Discovery

Komagataella pseudopastoris was first detected in four samples collected from willow trees (Salix alba) in Budapest and Dorog, Hungary.[1] The collected strains were phenotypically very similar to Komagataella pastoris (which was then classified as Pichia pastoris) but were genetically distinct. The species was originally named Pichia pseudopastoris, with the knowledge that it may be reclassified into the genus Komagataella, which was largely unaccepted at the time.[1] It was officially moved into Komagataella in 2005.[2]

Growth and morphology

Komagataella pseudopastoris cells, when grown on malt extract agar, have been described as "spheroid to ovoid", with lengths ranging from 4-5 μm.[1] Carbon sources used by K. pseudopastoris include xylose, trehalose, and rhamnose. Fermentation of glucose has been observed; no other common sugars are fermented by K. pseudopastoris. Growth occurs at 36°C, but not at 38°C. Cells produce one to four hat-shaped ascospores.[1]

Phylogeny

Within Komagataella, K. pseudopastoris is considered to be most closely related to K. populi and K. mondaviorum.[3][4]

References

  1. ^ a b c d Dlauchy, Dénes; Tornai-Lehoczki, Judit; Fülöp, László; Péter, Gábor (2003-06-01). "Pichia (Komagataella) pseudopastoris sp. nov., a new yeast species from Hungary". Antonie van Leeuwenhoek. 83 (4): 327–332. doi:10.1023/A:1023318829389. ISSN 1572-9699. PMID 12777068.
  2. ^ Kurtzman, Cletus P. (2005). "Description of Komagataella phaffii sp. nov. and the transfer of Pichia pseudopastoris to the methylotrophic yeast genus Komagataella". International Journal of Systematic and Evolutionary Microbiology. 55 (2): 973–976. doi:10.1099/ijs.0.63491-0. ISSN 1466-5034. PMID 15774694.
  3. ^ Heistinger, Lina; Dohm, Juliane C.; Paes, Barbara G.; Koizar, Daniel; Troyer, Christina; Ata, Özge; Steininger-Mairinger, Teresa; Mattanovich, Diethard (2022-04-25). "Genotypic and phenotypic diversity among Komagataella species reveals a hidden pathway for xylose utilization". Microbial Cell Factories. 21 (1): 70. doi:10.1186/s12934-022-01796-3. ISSN 1475-2859. PMC 9036795. PMID 35468837.
  4. ^ Naumova, E. S.; Boundy-Mills, K. L.; Naumov, G. I. (2020-05-01). "Phylogenetics and Biogeography of Biotechnologically Important Methylotrophic Yeasts Komagataella". Microbiology. 89 (3): 294–300. doi:10.1134/S002626172003011X. ISSN 1608-3237.