Komagataella mondaviorum
| Komagataella mondaviorum | |
|---|---|
| Scientific classification | |
| Domain: | Eukaryota |
| Kingdom: | Fungi |
| Division: | Ascomycota |
| Class: | Pichiomycetes |
| Order: | Pichiales |
| Family: | Pichiaceae |
| Genus: | Komagataella |
| Species: | K. mondaviorum
|
| Binomial name | |
| Komagataella mondaviorum Naumov, 2018
| |
Komagataella mondaviorum is a species of ascomycete fungi existing as a yeast.
Discovery
Komagataella mondaviorum was identified as a unique species in 2018 from strains collected throughout the 1950s and 1970s. The strains were previously believed to be Komagataella pastoris.[1] K. mondaviorum was declared a distinct species due to differences in the genome found during DNA sequencing.[1] It was named after Robert and Margrit Mondavi for their contributions to the California wine industry.[1]
Growth and morphology
Komagataella mondaviorum, when grown on malt extract agar, has been described as spherical or ovoid, with lengths ranging between 2-7 μm.[1] Cells are observed to grow singly or in pairs. Colonies are white and smooth. Hat-shaped ascospores have been observed.[1]
Glucose is fermented by K. mondaviorum; fermentation of other common sugars has not been observed.[1] No growth occurs at or above 35°C.[1][2]
Phylogeny
Within Komagataella, K. mondaviorum is considered to be most closely related to K. populi and K. pseudopastoris.[1][2][3]
Ecology and distribution
Komagataella mondaviorum has been isolated from various tree species, including cottonwood (Populus deltoides), black cottonwood (Populus trichocarpa), black oak (Quercus kelloggii), willow (Salix sp.), and hackberry (Celtis sp.).[1][3] It has been discovered in the United States (California, Washington, and Alaska) as well as Canada (British Columbia).[1][3]
Utility in industrial protein synthesis
Komagataella mondaviorum has been proposed as a promising candidate for protein synthesis at an industrial scale, with the potential to synthesize products more efficiently than commonly used Komagataella species such as K. phaffii.[4]
References
- ^ a b c d e f g h i j Naumov, Gennadi I.; Naumova, Elena S.; Boundy-Mills, Kyria L. (2018-07-01). "Description of Komagataella mondaviorum sp. nov., a new sibling species of Komagataella (Pichia) pastoris". Antonie van Leeuwenhoek. 111 (7): 1197–1207. doi:10.1007/s10482-018-1028-6. ISSN 1572-9699.
- ^ a b Heistinger, Lina; Dohm, Juliane C.; Paes, Barbara G.; Koizar, Daniel; Troyer, Christina; Ata, Özge; Steininger-Mairinger, Teresa; Mattanovich, Diethard (2022-04-25). "Genotypic and phenotypic diversity among Komagataella species reveals a hidden pathway for xylose utilization". Microbial Cell Factories. 21 (1): 70. doi:10.1186/s12934-022-01796-3. ISSN 1475-2859. PMC 9036795. PMID 35468837.
- ^ a b c Naumova, E. S.; Boundy-Mills, K. L.; Naumov, G. I. (2020-05-01). "Phylogenetics and Biogeography of Biotechnologically Important Methylotrophic Yeasts Komagataella". Microbiology. 89 (3): 294–300. doi:10.1134/S002626172003011X. ISSN 1608-3237.
- ^ Gordeeva, T. L.; Borschevskaya, L. N.; Feday, T. D.; Tkachenko, A. A.; Sineoky, S. P. (2022-12-01). "The Expression Potential of Novel Komagataella Strains". Applied Biochemistry and Microbiology. 58 (8): 916–922. doi:10.1134/S0003683822080038. ISSN 1608-3024.