Samuel H. Sternberg

Samuel H. Sternberg, PhD
NationalityAmerican
Alma materColumbia University (B.A. in Biochemistry, 2007); University of California, Berkeley (Ph.D. in Chemistry, 2014)
Known forCRISPR–Cas systems, discovery of CRISPR-associated transposons, genome engineering, science communication
AwardsNIH Director’s New Innovator Award; Alfred P. Sloan Research Fellowship; Pew Biomedical Scholar; (Other awards can be added)
Scientific career
FieldsBiochemistry, Molecular Biophysics, Genome Engineering
InstitutionsColumbia University, Department of Biochemistry and Molecular Biophysics
Doctoral advisorJennifer A. Doudna

Samuel H. Sternberg, PhD is an American protein–RNA biochemist and an expert on CRISPR–Cas gene editing technology. He is an Associate Professor in the Department of Biochemistry and Molecular Biophysics at Columbia University and an Investigator with the Howard Hughes Medical Institute. His research focuses on the molecular mechanisms of CRISPR–Cas systems and the development of genome engineering tools. In addition, Dr. Sternberg is known for his science communication efforts, including public lectures and media appearances discussing the ethical and societal implications of genome editing.

Early life and education

Sternberg was born and raised in Lancaster, Pennsylvania.[1] He earned his B.A. in biochemistry from Columbia University in 2007, graduating summa cum laude, and later received his Ph.D. in chemistry from the University of California, Berkeley in 2014, where he worked in the laboratory of Professor Jennifer A. Doudna.[2]

Career

After completing his doctoral studies, Sternberg worked briefly as a postdoctoral researcher and later as a Scientist and Group Leader at Caribou Biosciences, a biotechnology company specializing in genome engineering applications.[3] In February 2018, he began his independent academic career at Columbia University, where he holds an appointment in the Department of Biochemistry and Molecular Biophysics. His laboratory investigates the mechanisms of CRISPR–Cas systems, including the discovery of CRISPR-associated transposons, and develops new tools for genome engineering.[4]

Research and contributions

Sternberg’s work has significantly advanced our understanding of CRISPR–Cas systems. His doctoral and postdoctoral research focused on the mechanism of RNA-guided DNA targeting by CRISPR–Cas9, and his laboratory has further pioneered the discovery of CRISPR-associated transposons that enable RNA-guided DNA integration. His research has been published in high-impact journals such as Nature, Science, and Cell. In addition to his research publications, he is a co-author (with Jennifer Doudna) of the popular science book A Crack in Creation, which discusses the discovery and implications of CRISPR technology.[5]

Other contributions include:

  • Demonstrating the mechanism of RNA-guided DNA targeting by CRISPR–Cas9, elucidating how Cas9 interrogates and cleaves DNA with high specificity.[6]
  • Contributing to the discovery and characterization of CRISPR-associated transposons that enable RNA-guided DNA integration, thereby expanding the toolkit available for genome engineering.[7][8]
  • Developing methodologies to improve the specificity of CRISPR–Cas systems by investigating off-target effects and optimizing guide RNA design.[9]
  • Advancing genome engineering tools through structural studies that revealed RNA-mediated conformational activation of Cas9, thereby informing the rational design of improved CRISPR-based technologies.[10]

Media coverage and public engagement

Sternberg has been featured in various news outlets and interviews discussing the transformative potential and ethical implications of CRISPR technology. For example, his work and viewpoints have been covered in The New York Times, which highlighted the promise and challenges of genome editing.[11] He has also delivered a TEDMED talk on CRISPR’s impact on human health and ethics.[12] In addition, his insights on genome engineering have been featured on podcasts such as DNA Today.[13]

Awards and honors

Sternberg has received several awards that recognize his contributions to genome engineering and CRISPR research, including:

  • Howard Hughes Medical Institute Investigator[14]
  • NIH Director’s New Innovator Award[15]
  • Alfred P. Sloan Research Fellowship in Chemistry[16]
  • Pew Biomedical Scholar[2]
  • Additionally, he has been recognized as a Schaefer Research Scholar and has received other accolades throughout his career.

Selected publications

  • O’Connell, M.R., Oakes, B.L., Sternberg, S.H., East-Seletsky, A., Kaplan, M., Doudna, J.A. “Programmable RNA recognition and cleavage by CRISPR/Cas9.” Nature 516 (2014): 263–266.[17]
  • Sternberg, S.H., LaFrance, B., Kaplan, M., Doudna, J.A. “Conformational control of DNA target cleavage by CRISPR-Cas9.” Nature 527 (2015): 110–113.[18]
  • Sternberg, S.H., et al. “DNA interrogation by the CRISPR RNA-guided endonuclease Cas9.” Nature 507 (2014): 62–67.[19]

References

  1. ^ "Samuel H. Sternberg". Samuel H. Sternberg. Retrieved April 1, 2025.
  2. ^ a b "Samuel H. Sternberg, PhD". Columbia University Irving Medical Center. 5 March 2018. Retrieved April 1, 2025.
  3. ^ "Samuel H. Sternberg - Associate Professor at Columbia University". LinkedIn. Retrieved April 1, 2025.
  4. ^ "Expert Answers: Sam Sternberg on Genome Engineering". Inside Scientific. March 2024. Retrieved April 1, 2025.
  5. ^ Jennifer A. Doudna and Samuel H. Sternberg. A Crack in Creation: Gene Editing and the Unthinkable Power to Control Evolution. Houghton Mifflin Harcourt, 2017.
  6. ^ Sternberg, SH; Redding, S; Jinek, M; Greene, EC; Doudna, JA (2014). "DNA interrogation by the CRISPR RNA-guided endonuclease Cas9". Nature. 507 (7490): 62–67. Bibcode:2014Natur.507...62S. doi:10.1038/nature13011. PMC 4106473. PMID 24476820.
  7. ^ Klompe, SE; Vo, PLH; Halpin-Healy, TS; Sternberg, SH (2019). "Transposon-encoded CRISPR-Cas systems direct RNA-guided DNA integration". Nature. 571 (7764): 219–225. doi:10.1038/s41586-019-1323-z. PMID 31189177.
  8. ^ Dolgin, Elie (2025-05-15). "Powerful CRISPR system inserts whole gene into human DNA". Nature. doi:10.1038/d41586-025-01518-w. ISSN 1476-4687. PMID 40379954.
  9. ^ O’Connell, MR; Oakes, BL; Sternberg, SH; East-Seletsky, A; Kaplan, M; Doudna, JA (2014). "Programmable RNA recognition and cleavage by CRISPR/Cas9". Nature. 516 (7530): 263–266. Bibcode:2014Natur.516..263O. doi:10.1038/nature13769. PMC 4268322. PMID 25274302.
  10. ^ Jinek, M; Jiang, F; Taylor, DW; Sternberg, SH; Kaya, E; Ma, E; Anders, C; Hauer, M; Zhou, K; Lin, S; Kaplan, M; Iavarone, AT; Charpentier, E; Nogales, E; Doudna, JA (2014). "Structures of Cas9 endonucleases reveal RNA-mediated conformational activation". Science. 343 (6176): 1247997–1–11. doi:10.1126/science.1247997. PMC 4184034. PMID 24505130.
  11. ^ "Jennifer Doudna on CRISPR: An Interactive Interview". The New York Times. August 15, 2022. Retrieved March 31, 2025.
  12. ^ What if we could rewrite the human genome? – TEDMED 2015. TED. November 30, 2015. Retrieved 2025-03-31.
  13. ^ "CRISPR, DNA and Genomics: Dr. Samuel Sternberg Examines This Game-Changing Technology". CHC Radio. August 6, 2018. Retrieved April 1, 2025.
  14. ^ "Samuel H. Sternberg, PhD | Investigator Profile | 2024-Present". www.hhmi.org. Retrieved 2025-06-02.
  15. ^ "NIH Director's New Innovator Award winners announced". NIH News. 2020. Retrieved April 1, 2025.
  16. ^ "Columbia Faculty Awarded Sloan Fellowships". Columbia University News. 2020. Retrieved April 1, 2025.
  17. ^ O’Connell, MR; Oakes, BL; Sternberg, SH; East-Seletsky, A; Kaplan, M; Doudna, JA (2014). "Programmable RNA recognition and cleavage by CRISPR/Cas9". Nature. 516 (7530): 263–266. Bibcode:2014Natur.516..263O. doi:10.1038/nature13769. PMC 4268322. PMID 25274302.
  18. ^ Sternberg, SH; LaFrance, B; Kaplan, M; Doudna, JA (2015). "Conformational control of DNA target cleavage by CRISPR-Cas9". Nature. 527 (7576): 110–113. Bibcode:2015Natur.527..110S. doi:10.1038/nature15544. PMC 4859810. PMID 26524520.
  19. ^ Sternberg, SH; Redding, S; Jinek, M; Greene, EC; Doudna, JA (2014). "DNA interrogation by the CRISPR RNA-guided endonuclease Cas9". Nature. 507 (7490): 62–67. Bibcode:2014Natur.507...62S. doi:10.1038/nature13011. PMC 4106473. PMID 24476820.
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